PP88 No Further a Mystery
To accomplish this, the inventors produced a big plasmid (˜twelve kb) exchanging the p15a-based mostly origin of replication from the primase origin. This plasmid targets the lacZ gene (p1322, SEQ ID NO: 25) in addition to is made up of a chloramphenicol marker. as it was disregarded if the RBS toughness would need to generally be modified to dupl